DNA recovery after sequential processing of latent fingerprints on black polyethylene plastic.

Latent fingerprints on plastic substrates can be visualized by using sequential treatments to enhance the contrast between the fingerprint residues and underlying substrate; however, the extent to which these processes affect subsequent DNA analysis is mostly unknown. Latent fingerprints deposited on black plastic by one donor were visualized with single-process fingerprint powders (i.e., white powder, bichromatic powder, or bichromatic magnetic powder) or sequential treatments (i.e., laser → reflected ultraviolet imaging system (RUVIS) → CA fuming → RUVIS → Rhodamine 6G, Ardrox, and MBD (RAM) or CA fuming → RAM/laser → bichromatic magnetic powder). Samples were examined after the addition of each treatment. DNA was collected using cotton swabs, extracted, quantified, and amplified. DNA yields, peak heights, number of alleles obtained, and percentage of DNA profiles eligible for CODIS upload were examined. Latent fingerprints processed with the laser and up to three sequential treatments generated DNA profiles with significantly higher peaks heights than those of the untreated samples. Fingerprints processed with the laser and up to two sequential treatments generated DNA profiles with significantly more alleles. All methods beginning with laser enhancement generated more CODIS-eligible profiles. Additional research is needed to determine the extent to which initial laser enhancement impacts the success of downstream DNA profiling results. Although DNA profile development is not guaranteed due to the variable quantities of DNA contained within latent fingerprints, the selection of an appropriate latent fingerprint visualization method could maximize both fingerprint detection and the generation of CODIS-eligible DNA profiles.

DNA recovery after sequential processing of latent fingerprints on copy paper.

Forensic examiners must determine whether both latent fingerprint development and DNA profiling can be performed on the same area of an evidence item and, if only one is possible, which examination offers the best chance for identification. Latent fingerprints can be enhanced by targeting different components of fingerprint residues with sequential chemical treatments. This study investigated the effects of single-reagent and sequential latent fingerprint development processes on downstream DNA analysis to determine the point at which latent fingerprint development should be stopped to allow for DNA recovery. Latent fingerprints deposited on copy paper by one donor were developed using three sequential processes: 1,8-diazafluoren-9-one (DFO) â†’ ninhydrin â†’ physical developer (PD); 1,2-indanedione-zinc (IND-Zn) â†’ ninhydrin â†’ PD; and IND-Zn â†’ ninhydrin â†’ Oil Red O (ORO) â†’ PD. Samples were examined after the addition of each chemical treatment. DNA was collected with cotton swabs, extracted, quantified, and amplified. DNA yields, peak heights, number of alleles obtained, and percentage of DNA profiles eligible for CODIS upload were examined. DNA profiles were obtained with varying degrees of success, depending on the number and type of treatments used for latent fingerprint development. The treatments that were found to be the least harmful to downstream DNA analysis were IND-Zn and IND-Zn/laser, and the most detrimental treatments were DFO, DFO/laser, and PD. In general, as the number of treatments increase, the opportunities for DNA loss or damage also increase, and it is preferable to use fewer treatments when developing latent fingerprints prior to downstream DNA processing.

Massively parallel sequencing and STR analysis from partial bloody fingerprints enhanced with columnar thin films.

Fingerprint enhancement often includes either physical or chemical approaches, such as fingerprint powder or cyanoacrylate fuming, to improve the quality of a fingerprint for visualization and analysis. However, these methods become more complex when fingerprints are partial bloody, and these procedures may interfere with downstream DNA analysis. Columnar thin film (CTF) deposition is a type of nanotechnology that utilizes an evaporant material to enhance a fingerprint under low-pressure conditions. Short tandem repeat (STR) analysis is the traditional method employed in crime laboratories. When DNA is of poor quality and quantity, like that often obtained from fingerprints, little to no genetic information may be obtained. Single nucleotide polymorphisms (SNPs) may be used to glean additional information when STR analysis fails. In this pilot study, 100 partial bloody fingerprints were collected from two donors and deposited on five different crime scene substrates, in which half were enhanced with CTFs and were graded for quality by an IAI-certified latent fingerprint examiner. CTF-developed fingerprints, on average, had higher grades compared to non-developed partial bloody fingerprints. STR analysis using Fusion 6C was performed to assess inhibition from the evaporant materials, in which no inhibition was observed. Sequencing of SNPs using the Precision ID Identity Panel was also employed, in which genetic information that could not be obtained from STRs was acquired with SNPs. Various sample types (i.e. pristine, low quality, and contaminated) utilized in this project demonstrated the acceptable performance of the Precision ID Identity Panel.